Cationic lipid-mediated co-transfection of insect cells
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چکیده
منابع مشابه
Cationic lipid-mediated co-transfection of insect cells
Current co-transfection protocols use calcium phosphate precipitation for cultured insect (Sf-9) cells followed by multiple agarose overlays to purify recombinant baculoviruses (1). We report a more effective and convenient method that uses the cationic lipid N[1,1-(2,3-dioleyloxy)propoyl]-N,N,N,trimethylammonium chloride, or DOTMA (2, 3), for cotransfections of Sf-9 cells and uses 96-well micr...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.13.4033